Abstract:
There were few purposes for this lab. First,
we caught isopods in the wild and bought them back to the classroom, and we
just closely observed their behaviors and structures. Then we used isopods to
test three different scenarios: wet vs. dry, acid vs. dry, base vs. dry, to
find out which environment they preferred. We placed 10 pillbugs in different
environments and observed for 11.5 minutes.
The results were that the pillbugs preferred wet conditions over dry and
base over acid. In addition, the pillbugs also showed taxis behavior in all
scenarios.
Introduction:
Ethology
is the study of animal behavior. An
animal’s behavior is everything an animal does and how it does it. There are three types of behaviors: orientation, agonistic, and mating. Orientation behaviors take the animal to its preferred
environment. Taxis is an animal moves
toward or away from a stimulus. Taxis is
often observed in response to changes in light, heat, moisture, sound, or
chemicals. Kinesis is random movement
and doesn’t involve stimuli. Agnostic
behavior is when animals respond to each other in aggressive or submissive
movements. An example would be when the
hair on a dog’s back stands up when it is being aggressive. Mating behaviors are activities that involve
finding, courting, and mating with a member of the same species. An example would be a peacock fluffing up its
feathers to attract females. There are
differences between classical conditioning and operant conditioning. Classical
conditioning is a type of associative learning in which an
arbitrary stimulus is associated with a reward or punishment, but Operant
Conditioning is a type of associative learning in which an animal learns to
associate one of its behavior with a reward or punishment; also known as
trial-and-error learning.
Hypothesis:
When isopods are exposed to wet and dry
conditions, they will prefer to stay in the wet environment because they are
used to living in dark, moist environment.
When isopods are exposed to acid or base conditions, they will prefer
the acid conditions.
Materials:
Petri dish
Connected chamber
Water
Pipette
Filter paper
Timer
Magnifier
Paint brush
Vinegar (acid)
Ammonia (base)
Procedure:
1. Collect 10 isopods from the wild
2.
Place isopods in a Petri dish with a paintbrush and observe them for 10
minutes. Record observations that are characteristic to the bugs.
3.
Place a wet filter paper in one chamber and a dry one in the other.
4.
Put the isopods in and cover the chambers
5.
Start the stopwatch and record the number of isopods in each chamber every 30
seconds
6.
After 3 minutes, replace the wet filter paper with one with vinegar
7.
Repeat procedure 4-5
8.
After 3 minutes, replace the vinegar filter paper with one with ammonia
9.
Repeat procedure 4-5
Data
Time (minutes)
|
Number of isopods in wet chamber
|
Number of isopods in dry chamber
|
Notes
|
0
|
-
|
-
|
|
0.5
|
6
|
6
|
|
1.0
|
5
|
5
|
|
1.5
|
2
|
8
|
|
2.0
|
4
|
6
|
|
2.5
|
4
|
6
|
|
3.0
|
4
|
6
|
|
3.5
|
3
|
7
|
|
4.0
|
3
|
7
|
|
4.5
|
3
|
7
|
|
5.0
|
3
|
7
|
|
Time (minutes)
|
Number of isopods in acidic chamber
|
Number of isopods in dry chamber
|
5.5
|
1
|
9
|
6.0
|
0
|
10
|
6.5
|
0
|
10
|
7.0
|
0
|
10
|
7.5
|
0
|
10
|
8.0
|
0
|
10
|
8.5
|
0
|
10
|
Time (minutes)
|
Number of isopods in base chamber
|
Number of isopods in dry chamber
|
9.0
|
1
|
9
|
9.5
|
3
|
7
|
10.0
|
4
|
6
|
10.5
|
4
|
6
|
11.0
|
5
|
5
|
11.5
|
6
|
4
|
Analysis:
The independent
variables were the different conditions in the chambers. For the first experiment, the independent
variables were the wet and dry conditions.
In the second and third experiment, the independent variables were the ph. For both experiments, the dependent variable
was the isopod behavior. The main
control for this experiment was the initial observation. The kinesis observed at this time was used to
determine if the isopod behavior in the two experiments was truly taxis behavior
in response to the different conditions.
Other controls include using the same amount of bugs, using a timer,
using the same chamber, and using similar textures in the chambers.
In the first
experiment, our data shows that isopods preferred the dry conditions. This is not what I expected, because pillbugs
suppose live in dark and moist environment and they use gills, so they need
moist conditions to survive. This could
have been due to different wet conditions.
The isopods may avoid an area if it is too wet and this would skew the
results.
In the second experiment, the isopods
preferred the dry conditions over the acid conditions. My hypothesis is wrong again, they do not
prefer to live in a more acidic environment. My initial thought was that they
live in soil and under logs, which are acidic conditions. However, it could be another experimental
error, because the vinegar was too acidic, they would rather stay in the dry chamber.
Conclusion:
From our
data, the isopods preferred the dry environment which is against their normal living
habit. My hypothesis was proven incorrect.
In the acid
vs. dry and base vs. dry experiment, the isopods preferred the base condition
which is again against their normal living habit. Most soils areas are acidic. The isopods did not choose the environment
that was closest to their natural habitat. My hypothesis was proven incorrect
again.